Question 1

How many units is a 25 mg dose of NAD+ (500mg vial, 2ml diluent)?

Accepted Answer

10 units on a standard U-100 insulin syringe (0.1 ml). Mixing a 500mg vial of NAD+ with 2ml of bacteriostatic water gives a concentration of 250 mg/ml. On a U-100 insulin syringe that means: 25 mg = 10 units · 50 mg = 20 units · 100 mg = 40 units.

Question 2

What is the concentration of 500mg of NAD+ in 2ml?

Accepted Answer

500mg ÷ 2ml = 250 mg/ml.

Question 3

Which syringe do these unit marks refer to?

Accepted Answer

A standard U-100 insulin syringe, where 100 units = 1 ml. On U-50 or U-30 syringes the same volume reads as half or 30% of the U-100 marks. These are mathematical conversions for organizational purposes only — not medical advice.

Dose	Draw volume	U-100 units
25 mg	0.1 ml	10 U
50 mg	0.2 ml	20 U
100 mg	0.4 ml	40 U

NAD+ 500mg in 2ml — syringe units chart